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KMID : 0861020160310010033
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Korea Journal of Herbology
2016 Volume.31 No. 1 p.33 ~ p.40
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Hepatoprotective effect of Paeoniae radix via Nrf2 activation
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Lee Soo-Hwan
Jung Ji-Yun
Park Sang-Mi
Jegal Kyung-Hwan
Byun Sung-Hui
Cho Il-Je
Kim Sang-Chan
Kim Kwang-Joong
Kim Young-Woo
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Abstract
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Objectives : Liver is one of the largest organs in the human, and has a function of detoxification and energy sensing to prevent severe disease. Paeoniae radix has been used to treat a variety of liver diseases such as hepatitis and chronic hepatic failure. Although P. radix has been used as an medicinal herb for a long time, the effects of P. radix on severe oxidative stress and its action mechanism on the liver was not clearly verified.
Methods : This study investigated the protective effects of P. radix extract (PRE), and the underlying mechanism of its action in the liver. tert-butyl hydroperoxide (t-BHP) and carbon tetrachlroride (CCl4) were used to induce oxidative stress in the HepG2 hepatocyte cell line and Sprague-Dawley rats, respectively.
Results : t-BHP significantly induced cell death and ROS production in HepG2 cell, as indicated by MTT and FACS analysis. However, pretreatment of PRE inhibited a decrease in cell viability and H2O2 production in the HepG2 cells. PRE also blocked the ability of t-BHP to damage in mitochondrial membrane transition. More importantly, PRE induced Nrf2 activation and antioxidant Phase II enzyme, which may have a role in the effects of PRE. In mice, PRE inhibited the liver damage induced by CCl4.
Conclusions : PRE inhibited oxidative stress and hepatic damages as mediated with Nrf2 activation. This study unveil, in part, the effect and mechanism of old medicinal herb, P. radix.
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KEYWORD
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Paeoniae radix, Oxidative stress, Nrf2 activation, Liver
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